Available from handbook.cochrane.org
For RSL3 treatment, 1 10 6 A549 cells were suspended in 100 l PBS and growth factor-reduced Matrigel (1:1) (Corning 356231) and injected subcutaneously (left and right flank) into NSG mice
The extraction was done in a buffer [1.0 mm ethylenediaminetetraacetic acid (EDTA), 100 mM TrisHCl (pH 7.3), 100 mM -mercaptoethanol, 1.0 mM phenyl methyl sulfonyl fluoride, 0.1 mM protease inhibitor cocktail, 2% glycerol, 25 mM MgCl 2 , 10 mM dipotassium hydrogen phosphate (K 2 HPO 4 )] and centrifuged at 12,000 g for 15 min, 4 C
J Physiol Anthropol
Amyloid- impairs mitochondrial dynamics and autophagy in Alzheimers disease experimental models
Mostly not approved, and the safety data is limited